Establishment of a Multiplex – PCR protocol for detection of Y chromosome microdeletion in Azoospermia male patients
نویسندگان
چکیده
منابع مشابه
Multiplex PCR Screening of Y Chromosome Microdeletions in Azoospermic Patients
Objective To develop a multiplex PCR protocol for routine screening of microdeletions on the Y chromosome Methods Five multiplex sets were established and Y chromosome microdeletions screening were carried out in 26 azoospermic men who undertook ICSI and 30 azoospermic men who undertook testicular biopsy. Results In 56 azoospermic men, 5 patients were found with AZFc/DAZ microdeletions, 2 patie...
متن کاملبررسی مولکولی حذف‌های کروموزوم Y در نواحی AZF بیماران مبتلا به آزواسپرمی و اولیگواسپرمی غیر انسدادی مراجعه‌کننده به مرکز ناباروری منتصریه مشهد
Background and Aim: The Y-chromosome azoospermic factor (AZF) regions consist of genes whose specific roles and functions in spermatogenesis and fertility have not been completely clarified. Hence, recognition of the association between AZF microdeletions and male infertility has suggestions for the diagnosis, treatment, and genetic counseling. The main objective of the present study was invest...
متن کاملmultiplex pcr based screening for microdeletions in azoospermia factor region of y chromosome in azoospermic and severe oligozoospermic south indian
background: y chromosomal microdeletion is an important genetic disorder, which may arise due to intrachromosomal recombination between homologous sequences in the male specific region of the human y chromosome. it is frequently associated with the quantitative reduction of sperm. the screening for y chromosomal microdeletions has a great clinical value. objective: to develop a sequence tagged ...
متن کاملLoss of the AZFc region due to a human Y-chromosome microdeletion in infertile male patients.
Infertility is a major reproductive health threat; the frequency of male infertility due to Y-chromosome microdeletions is 13-18% in the human population; these microdeletions involve recurrent loss of three non-overlapping regions designated as AZFa, AZFb and AZFc, associated with spermatogenic failure. Several contradictory reports have been published regarding deletion frequency based on seq...
متن کاملMultiplex-Polymerase Chain Reaction for Detecting Microdeletions in The Azoospermia Factor Region of Y Chromosome in Iranian Couples with Non-Obstructive Infertility and Recurrent Pregnancy Loss
Objective Approximately 15 percent of couples are infertile. The male factor is responsible for approximately 50% of the cases. One of the main genetic factors playing a role in male infertility is Y chromosomal microdeletion within the proximal long arm of the Y chromosome (Yq11), named azoospermia factor (AZF) region. Recent studies have also demonstrated that there is a potential connection ...
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ژورنال
عنوان ژورنال: Science and Technology Development Journal
سال: 2015
ISSN: 1859-0128,1859-0128
DOI: 10.32508/stdj.v18i4.904